RATIONALE: In Israel the majority of infants less than 12 months old regularly consume peanut products in contrast to the UK where infants avoid peanut products. Previous studies suggest a low prevalence of peanut allergy in Israel and raise the possibility that the processing of Israeli peanut snacks renders them hypoallergenic. Therefore, the allergen content and allergenicity of the most popular peanut products consumed by children and infants in the UK and Israel were compared to determine if such differences could explain the variation in the prevalence of peanut allergy. METHODS: The total protein content of whole snack products was determined using LECO analysis and various immunoassays were utilized to determine the percentage of peanut protein in each product. The products were all normalized according to peanut protein content and subjected to SDS-PAGE, Western blot and Slot blot analysis with anti-peanut, anti-Ara h 1, 2 and 3 antibodies and pooled serum from peanut allergic individuals. RESULTS: Peanut protein levels from Israeli and U.K. products were found to be between 68-100%. The Ara h 1, Ara h 2 and Ara h 3 proteins in each peanut product were intact and the levels were comparable. Similarly, IgE binding analysis with pooled serum from 9 allergic individuals was nearly identical when the same amount of peanut protein was used for each product. CONCLUSIONS: The contents of peanut protein, individual major allergens and IgE binding capacity of the popular snacks from Israel cannot explain the large discrepancies in the prevalence of peanut allergy among the two countries. Funding: National Peanut Board and the USDA
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机译:理由:在以色列,大多数小于12个月的婴儿经常食用花生产品,而英国则避免食用花生产品。先前的研究表明,以色列的花生过敏症患病率较低,并增加了以色列花生点心的加工使其具有低变应原性的可能性。因此,比较了英国和以色列儿童和婴儿所消费的最受欢迎的花生产品的过敏原含量和过敏原性,以确定这种差异是否可以解释花生过敏症患病率的差异。方法:使用LECO分析法测定整个零食产品的总蛋白质含量,并通过各种免疫测定法确定每种产品中花生蛋白质的百分比。将所有产品根据花生蛋白含量进行标准化,并进行SDS-PAGE,Western印迹和Slot印迹分析,分别使用抗花生蛋白,抗Ara h 1、2和3抗体以及来自花生过敏个体的合并血清。结果:发现以色列和英国产品的花生蛋白水平在68-100%之间。每个花生产品中的Ara h 1,Ara h 2和Ara h 3蛋白均完整无损,且水平相当。同样,当每种产品使用相同量的花生蛋白时,使用来自9个过敏性个体的合并血清的IgE结合分析几乎相同。结论:以色列流行小吃的花生蛋白含量,主要主要变应原和IgE结合能力不能解释这两个国家花生过敏发生率的巨大差异。资金来源:国家花生委员会和美国农业部
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